Hands-On Training on PCR, Gel Electrophoresis, and PGPR Traits

02 December to 05 December 2024

Workshops are a vital tool for bridging the gap between theoretical knowledge and practical expertise. This exclusive workshop focuses on hands-on training in three crucial techniques in molecular biology and microbiology: Polymerase Chain Reaction (PCR), Gel Electrophoresis, and the evaluation of Plant Growth-Promoting Rhizobacteria (PGPR) traits. These techniques play a foundational role in research and applied sciences, particularly in fields like genetics, molecular biology, biotechnology, and sustainable agriculture..

Polymerase Chain Reaction (PCR): The DNA Amplification Tool

PCR is a revolutionary technique that allows for the amplification of specific DNA sequences. This method, introduced by Kary Mullis in 1985, has transformed molecular biology by enabling researchers to replicate DNA in vitro.

❖ Why Learn PCR?
PCR is widely used in various applications, including genetic fingerprinting, disease diagnosis, and evolutionary biology. Participants in this workshop will learn the step-by-step process of PCR, from preparing reaction mixtures to programming thermal cyclers.

❖ Hands-On Activities:
Attendees will:
➢ Isolate DNA from biological samples.
➢ Prepare and optimize PCR reactions.
➢ Analyze amplification results using gel electrophoresis

❖ Key Outcomes:
By the end of the session, participants will have a clear understanding of primer design, thermal cycling, and troubleshooting PCR issues.

Gel Electrophoresis: Visualizing DNA Fragments:

Gel electrophoresis is a technique used to separate DNA fragments based on their size. It is an essential tool for visualizing and analyzing PCR products, making it an indispensable complement to PCR.

❖ Why Learn Gel Electrophoresis?
This technique helps in verifying the success of PCR, identifying genetic markers, and separating nucleic acids or proteins for further studies. It is a must-know skill for researchers in life sciences.

❖ Hands-On Activities:
Participants will:
➢ Prepare agarose gels.
➢ Load and run DNA samples
➢ Use staining techniques to visualize DNA under UV light

❖ Key Outcomes:
Attendees will gain the ability to interpret band patterns on a gel, identify molecular weight markers, and understand electrophoretic mobility principles..

Plant Growth-Promoting Rhizobacteria (PGPR) Traits

PGPR are beneficial bacteria that colonize plant roots and enhance plant growth by various mechanisms. Their importance in sustainable agriculture and environmental biotechnology and microbiology has garnered significant attention

❖ Why Focus on PGPR Traits?
With the growing demand for eco-friendly agricultural solutions, studying PGPR traits such as nitrogen fixation, phosphate solubilization, and production of phytohormones has become crucial. This session will introduce participants to methods for isolating and characterizing PGPR, along with evaluating their functional traits

❖ Hands-On Activities:
Participants will:
➢ Isolate rhizobacteria from soil samples
➢ Perform biochemical assays to assess PGPR traits like indole-3-acetic acid (IAA) production and phosphate solubilization.
➢ Interpret results and discuss their implications for agricultural productivity

❖ Key Outcomes:
By the end of this session, participants will be equipped to work with PGPR, evaluate their traits, and understand their application in sustainable farming practices..

Why Attend This Workshop?
This workshop provides a unique combination of molecular biology and microbiology techniques. Participants will gain:
Practical Skills: Hands-on training ensures that attendees can confidently perform PCR, run electrophoresis gels, and assess PGPR traits.
Interdisciplinary Knowledge :The integration of molecular and microbial techniques opens up opportunities in diverse fields, from healthcare to agriculture.
Career Advancement : Practical knowledge of these techniques is highly valued in academic research, biotechnology companies, and agricultural industries.
Networking Opportunities :Interact with experts and peers to exchange ideas, seek guidance, and foster collaborations.

Who Should Attend?
This workshop is ideal for:
● Students and researchers in biotechnology, molecular biology, microbiology.
● Professionals seeking hands-on exposure to these fundamental techniques.
● Anyone interested in advancing their skills in molecular and microbial research.

Conclusion of this workshop:
The Hands-On Training: PCR, Gel Electrophoresis, and PGPR Traits workshop is a one-of-a-kind opportunity to master essential laboratory techniques. By combining theoretical sessions with practical exposure, this workshop ensures that participants leave with the confidence and skills to apply these methods in real-world scenarios. Whether you are a budding researcher or a professional seeking to upgrade your expertise, this workshop offers the tools you need to excel in your field.